At first, bones found in the Bluefish Caves and at a mine site in northern Yukon were assumed to belong to an ice-age puma or cougar.
But DNA analysis from the University of California said otherwise.
The test was performed by Molly Cassatt-Johnstone, at the time a lab technician who had just finished her undergrad. The Yukon bones were some of the first samples she ever worked on.
“So when I did the first preliminary analyses, and these didn’t look like pumas at all, I was like, ‘Oh, I’ve done something really, really wrong here,’” she laughed.
Yukon paleontologist Grant Zazula still remembers his amazement at the message he received that evening: “Hey Grant, your cougars aren’t cougars — they’re actually cheetahs.”
“Twenty-five years ago, looking at ancient DNA was almost science fiction, Jurassic Park kind of stuff,” said Zazula. “Now, doing genetic analysis on permafrost-preserved bones from the Yukon is kind of a regular thing we do.”
Cassatt-Johnstone had become the first to identify a Yukon Miracinonyx — the creature also known as the extinct American cheetah. Now, she is the first author of a paper published in Current Biology that sheds new light on the ice-age cat’s diet and range.
Researchers long believed the American cheetah, with the long limbs and slender build of today’s living cheetahs, would have evolved to chase pronghorns across grassy plains. To find them in the middle of Arctic tundra threw paleontologists for a loop.
“They were originally thought to be like really close, specialized predators of American pronghorn antelope in the lower 48,” said Cassatt-Johnstone. “But there were never any pronghorn in Beringia.”
Beringia is the place where Asia and North America were once connected, and includes the land now part of Russia's easternmost region, Alaska and the Yukon.
To answer that question, the study authors, which include collaborators from Canada and the U.S., turned to stable isotope analysis.
The technique, also used in forensics, looks for chemical clues to investigate what ancient people or animals were eating, and can even be used to track things like the climate they were living in and where they may have travelled in their lifetime.
Slivers of bone that would have once formed part of the cheetah’s paw and leg were analysed through a process Matthew Wooller says is precise enough to be able to tell if someone has eaten plain carrots or carrot cake.
Wooller, the director of the Alaska Stable Isotope Facility at the University of Alaska Fairbanks, said he was determined to crack the case after reviewing preliminary results.
“I was hooked,” he said. “The data was like nothing I’d seen before, very unusual data from an isotope perspective.”
